After removing batch effects, we used all rsRNA to reduce the dimensionality of all samples from nine tissues to two-dimensional from high dimensionality using the t-SNE method. The distribution of various types of tissues is scattered, and the samples of the same tissue are clustered together, indicating that rsRNA is tissue-specific and can distinguish different tissue types. Then, the differential rsRNA of each of the nine tissues relative to the other eight tissues was calculated, and the top 10 significantly highly expressed rsRNA in each tissue were selected to draw the heat map. We can clearly see that different tissues have their own highly expressed rsRNA, and these marker rsRNA are tissue-specific and differentially expressed in different tissues.